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Basic Techniques of Genetic Engineering - opis przedmiotu

Informacje ogólne
Nazwa przedmiotu Basic Techniques of Genetic Engineering
Kod przedmiotu 13.9-WB-P-BTGE-S14
Wydział Wydział Nauk Biologicznych
Kierunek WNB - oferta ERASMUS
Profil -
Rodzaj studiów Program Erasmus
Semestr rozpoczęcia semestr zimowy 2019/2020
Informacje o przedmiocie
Semestr 1
Liczba punktów ECTS do zdobycia 6
Typ przedmiotu obowiązkowy
Język nauczania angielski
Sylabus opracował
  • dr Ewa Bok
Formy zajęć
Forma zajęć Liczba godzin w semestrze (stacjonarne) Liczba godzin w tygodniu (stacjonarne) Liczba godzin w semestrze (niestacjonarne) Liczba godzin w tygodniu (niestacjonarne) Forma zaliczenia
Wykład 15 1 - - Egzamin
Laboratorium 45 3 - - Zaliczenie na ocenę

Cel przedmiotu

The aim of the course is to present issues related to techniques of manipulation of genetic material. The aim of the laboratory classes is to familiarize the students with rules for safe work in the molecular biology laboratory and to acquire by the students the practical knowledge concerned the basic techniques of genetic engineering.

Wymagania wstępne

Knowledge of the biochemistry, general and molecular genetics and microbiology courses.

Zakres tematyczny

Lecture: The term “genetic engineering”. To what purpose there are used the techniques of genetic engineering? The most important discoveries in molecular biology. Methods of isolation and purification of DNA. Restriction enzymes - the basic tool of genetic engineering. Another enzymes used in cloning. Vectors used in cloning experiments, - choice of the vector type appropriate to host cells. Methods used to transfer DNA into prokaryotic and eukaryotic cells. Cloning strategies. Generation of genomic and cDNA libraries. Selection and screening of recombinants.

Laboratory classes: - preparation of vector for cloning – isolation and purification of plasmid DNA, -  principles of nucleic acid separation by agarose gel electrophoresis, - preparation of competent cells, - preparation of the DNA insert for cloning, - ligation, - transformation of competent cells, -  restriction analysis of recombinant plasmids, - expression and purification of recombinant protein, - overexpression analysis by SDS–PAGE.

Metody kształcenia

Lecture in the form of multimedia presentations. Laboratory classes in the classroom equipped with the appropriate analytical apparatus and equipment.

Efekty kształcenia i metody weryfikacji osiągania efektów kształcenia

Opis efektu Symbole efektów Metody weryfikacji Forma zajęć

Warunki zaliczenia

The lectures – the requirement of the assessment is to get pass mark credit of written examination, which lasts 90 minutes. The examination test contains 30 questions (open and closed), 60% of all of the points are required to get the pass mark credit. Laboratory classes - the requirements of the assessment criteria refer to the attendance at classes, the active participation in laboratory experiments and to get the pass mark credit of written tests with open and closed questions (above 60% of all of the points are required to get the pass mark credit) and credit of all written reports from performed laboratory experiments. The final mark consists of the average sum of all of the pass partial marks.

Literatura podstawowa

  1. Nicholl D., An Introduction to Genetic Engineering, Cambridge University Press, 2008.
  2. White M.R.H., Turner P.C., McLennan A.G. & Bates A.D, Instant Notes in Molecular Biology, Bios, Oxford, 2012.
  3. Primrose S.B., Twyman R. Principles of Gene Manipulation and Genomics, Wiley-Blackwell, 2006.

Literatura uzupełniająca

  1. Selected current research and review articles.

Uwagi


Zmodyfikowane przez dr Ewa Bok (ostatnia modyfikacja: 01-05-2019 23:17)