1. SylabUZ
2. Wydział Lekarski i Nauk o Zdrowiu
3. semestr zimowy 2023/2024
4. WLiNZ - oferta ERASMUS / Lekarski - jednolite magisterskie sześcioletnie
5. Molecular Biology

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Molecular Biology - opis przedmiotu

Cel przedmiotu

The primary objective of teaching medical and molecular biology is integrating knowledge of fundamental disciplines, giving rise to the study of clinical genetics. Knowledge of the human genome organisation and mechanisms of gene expression regulation, mutagenesis and DNA repair processes enable a better understanding of the aetiology of many diseases. Learning about the mechanisms of gene expression, mutagenesis and DNA repair processes is needed to understand many human diseases. This course allows familiarisation with the methods of molecular biology and the possibilities of their application in genetic research.

Wymagania wstępne

Zapisz zmiany

Knowledge in the field of organic chemistry, biochemistry and cell biology.

Zakres tematyczny

Lecture

1. Structure prokaryotes and eukaryotes cells.

2. Structure and function of genetic material (DNA, RNA).

3. Replication, transcription and translation.

4. The regulation of gene expression.

5. Organization of the human genome.

6. Variability of DNA, mutations, and DNA repair mechanisms.

7. Molecular basis of neoplastic diseases.

Seminars

1. Basics of genomics and cytogenetics. Human genome.

2. Principles of inheritance and the basics of genetic variation. Genetic variability in the population and estimation of the appearance of a given trait.

3. Mutations - types and mutagenic factors, the influence of drugs, chemical compounds, physical factors, and environmental pollution.

4. Selected human diseases with autosomal and X-linked autosomal inheritance, structural and numerical aberrations.

5. Types of changes detected in DNA / RNA, methods of detecting known mutations.

6. Methods for detecting mutations and polymorphisms in DNA, RNA: reverse transcription, hybridization techniques – Southern and Northern blot, oligonucleotide microarrays, analysis of gene expression using cDNA microarrays, fluorescence in situ hybridization (FISH), SNG, use of multiple fluorescent probes in real-time PCR, MALDI-TOF mass spectrometry

7. Ecogenetics, pharmacogenetics. Laboratories

Laboratory classes

1. Basic work principles in a molecular biology laboratory and with laboratory equipment. Basic techniques for isolating DNA and RNA nucleic acids and types of electrophoretic separations of nucleic acids.  

2. Isolation of lymphocytes from the blood. Isolation of genomic DNA by column method. DNA electrophoretic separation and analysis of results. Determination of DNA concentration and purity.

3. Principles and types of PCR reactions. Designing primers for PCR reactions. Electrophoretic analysis of PCR products, separation results analysis, and conclusions formulation.

4. Study of gene polymorphisms. Genotyping with the use of PCR-RFLP and Real-Time PCR methods. Calculating the copies number of DNA.

5. Principles of designing and conducting scientific research, interpretation of results, and conclusions.

Metody kształcenia

Lectures in the form of multimedia presentations. Seminars in the form of multimedia presentations and discussions.  
Laboratory classes include a practical presentation of particular stages of experiments and the student's active participation in the genetic material preparation. Students learn to use specialised equipment for molecular genotyping in medical diagnostics and scientific research.

Efekty uczenia się i metody weryfikacji osiągania efektów uczenia się

Opis efektu	Symbole efektów	Metody weryfikacji	Forma zajęć

Warunki zaliczenia

Laboratory classes: pass based on attendance and activity in the course and one summary test including 25 single-choice test questions and five open questions - positive grade for 60% of the points obtained. During the laboratory classes, one justified absence is allowed, which must be made up as a written test covering three questions on the thematic scope of the laboratory classes within the time limit agreed with the teacher.

Seminar classes: pass based on attendance and participation in classes and partial tests checking knowledge after each seminar - positive grade from 60% of the points obtained. The credit is the arithmetic mean of the partial rates of the above classes. During the seminar classes, one excused absence is allowed (sick leave). Absences, in each case, must be made up for in the form of a written test or an oral answer covering the scope of the material for the seminar classes within the time limit agreed with the teacher.

Final exam from the course: The condition for admission to the examination is positive completion of the controlled classes (seminar and laboratory exercises). The final exam in molecular biology is written (60 test questions) and covers the full range of the subject material (lecture, seminar, laboratory exercises). The student must get at least 36 points (60%) for a positive exam result.

Final grade: arithmetic mean of all forms provided for the course. The mean results are determined according to the principle: mean 3.25 is the definitive mark of 3.5; mean 3.75 is the final score of 4.0; mean 4.25 is the final score of 4.5; the mean of 4.75 is the final score of 5.0.

Literatura podstawowa

1. Terry A. Brown, Genomes, LA, 2010.

2. Primros E. Architecture of the human genome, NY, 2000. 

Literatura uzupełniająca

Uwagi

Zmodyfikowane przez dr hab. Katarzyna Baldy-Chudzik, prof. UZ (ostatnia modyfikacja: 11-05-2023 14:04)