1. SylabUZ
2. Wydział Nauk Ścisłych i Przyrodniczych
3. semestr zimowy 2023/2024
4. WNB - oferta ERASMUS - Program Erasmus
5. Basic techniques of genetic engineering

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Basic techniques of genetic engineering - opis przedmiotu

Cel przedmiotu

The lecture on Basic Genetic Engineering Techniques is designed to introduce students to issues in the field of techniques used to manipulate genetic material, various DNA cloning techniques and recombinant DNA techniques. Laboratory classes are designed to introduce students to the principles of safe work in a molecular biology laboratory and to provide practical knowledge of basic genetic engineering techniques. The student should acquire the ability to perform the cloning experiment and develop the ability of critical analysis and proper interpretation of the results.

Wymagania wstępne

Zapisz zmiany

Knowledge of the biochemistry, general and molecular genetics and microbiology courses.

Zakres tematyczny

Lecture: Goals and tasks of genetic engineering. Benefits and risks of gene manipulation. Genetic engineering in the light of moral and ethical issues. Methods of introducing DNA into prokaryotic and eukaryotic cells. Restriction enzymes as a basic tool in genetic engineering. Other enzymes used in genetic engineering and DNA analysis. Cloning - vectors used for cloning and their selection. Cloning strategies. Creating genomic and cDNA libraries. Selection and screening of positive clones.

Laboratory classes: Preparation of the plasmid vector, isolation and purification of plasmid DNA. Preparation of competent cells for DNA cloning. Transformation of competent cells. Selection and analysis of clones. Restriction analysis of recombinant plasmids.

Metody kształcenia

Lecture in the form of multimedia presentations. Laboratory classes in the classroom equipped with the appropriate analytical apparatus and equipment.

Efekty uczenia się i metody weryfikacji osiągania efektów uczenia się

Opis efektu	Symbole efektów	Metody weryfikacji	Forma zajęć

Warunki zaliczenia

The lectures – the requirement of the assessment is to get pass mark credit of written examination, which lasts 60 minutes. The examination test contains 30 questions (open and closed), 60% of all of the points are required to get the pass mark credit. Laboratory classes - the requirements of the assessment criteria refer to the attendance at classes, the active participation in laboratory experiments and to get the pass mark credit of written tests with open and closed questions (above 60% of all of the points are required to get the pass mark credit) and credit of all written reports from performed laboratory experiments. The final mark consists of the average sum of all of the pass partial marks.

Literatura podstawowa

1. Nicholl D., An Introduction to Genetic Engineering, Cambridge University Press, 2008.
2. White M.R.H., Turner P.C., McLennan A.G. & Bates A.D, Instant Notes in Molecular Biology, Bios, Oxford, 2012.
3. Primrose S.B., Twyman R. Principles of Gene Manipulation and Genomics, Wiley-Blackwell, 2006.

Literatura uzupełniająca

1. Selected current research and review articles.

Uwagi

Zmodyfikowane przez dr Elżbieta Heger (ostatnia modyfikacja: 26-04-2023 08:13)