SylabUZ
| Course name | General enzymology |
| Course ID | 13.4-WB-P-GEn-S21 |
| Faculty | Faculty of Exact and Natural Sciences |
| Field of study | WNB - oferta ERASMUS |
| Education profile | - |
| Level of studies | Erasmus programme |
| Beginning semester | winter term 2024/2025 |
| Semester | 2 |
| ECTS credits to win | 5 |
| Course type | obligatory |
| Teaching language | english |
| Author of syllabus |
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| The class form | Hours per semester (full-time) | Hours per week (full-time) | Hours per semester (part-time) | Hours per week (part-time) | Form of assignment |
| Laboratory | 30 | 2 | - | - | Credit with grade |
| Lecture | 20 | 1,33 | - | - | Credit with grade |
Gaining knowledge about the classification of enzymes. Understanding the mechanism of action of enzymes. Understanding the basics of enzyme kinetics and the importance of the parameters describing this kinetics. Understanding of the mechanisms of activity control of the enzyme, its activation and inhibition. Familiarization with impact of different types of inhibition to change of enzyme kinetic parameters. Gaining knowledge to plan and carry out enzymatic tests.
Basic knowledge about the structure of proteins. Skill of using computer and Internet.
Definition and general characteristics of enzymes. Characteristics of the different classes of enzymes. The role and mechanism of enzyme action. EC enzyme codes. Fundamentals of enzymatic kinetics. Analysis of enzymatic reaction. The Michaelis-Menten equation. The basic parameters describing the kinetics of enzyme - the maximum rate of the enzymatic reaction and Michaelis constant. Identification of enzymes (metabolic pathways). Characteristics of selected enzymes and metabolic pathways and their control. Energy states during the enzymatic reaction. Important coenzymes and their precursors. The Lineweaver-Burk formula - the purpose and significance of such formula conversion. Planning and carrying out enzymatic test. Calculation of the kinetic parameters from the data obtained from enzymatic test. Activation and control of enzymatic activity. Allosteric enzymes. Allosteric reaction kinetics. Cascade-enzymatic reactions. Factors affecting enzyme activity. Characteristics of inhibition effects. Enzymatic reactions with the competitive, acompetitive, noncompetitive, mixed, substrate and product inhibition. Analysis of enzymatic inhibition kinetics.
Lectures with multimedia presentations. Laboratories: using online enzymatic databases and specialized software, virtual enzymatic tests, development of topics related to the enzymology.
| Outcome description | Outcome symbols | Methods of verification | The class form |
Lecture - written colloquium consisting of 10 questions. The correct answers to at least 6 questions (60%) are required to get the pass mark credit.
Laboratory assessment is evaluated on the basis of development and presentation of topics related to the enzymology, for example characterization and identification of selected enzymes, and demonstration of the ability to perform virtual enzymatic tests. The final grade from the laboratory is the arithmetic mean of the grades obtained from the development and presentation of the above issues.
1. Berg J.M., Tymoczko J.L., Stryer L., Biochemistry: International Edition, 2011.
2. Murray R.K., Bender D.A., Botham K.M., Kennelly P.J., Rodwell V.W., Weil P.A., Harper's Illustrated Biochemistry, Twenty-Eighth Edition, McGraw-Hill Companie, 2009.
Garret R.H, Grisham C.M., Biochemistry, Thomson Learning, 2012.
Modified by dr hab. inż. Andrzej Kasperski, prof. UZ (last modification: 27-03-2024 17:29)
