SylabUZ
| Course name | Biochemistry |
| Course ID | 13.6-WB-OS2P-B-ch-S17 |
| Faculty | Faculty of Biological Sciences |
| Field of study | Environmental Protection |
| Education profile | academic |
| Level of studies | First-cycle studies leading to Bachelor's degree |
| Beginning semester | winter term 2021/2022 |
| Semester | 3 |
| ECTS credits to win | 7 |
| Course type | obligatory |
| Teaching language | english |
| Author of syllabus |
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| The class form | Hours per semester (full-time) | Hours per week (full-time) | Hours per semester (part-time) | Hours per week (part-time) | Form of assignment |
| Lecture | 35 | 2,33 | - | - | Exam |
| Laboratory | 45 | 3 | - | - | Credit with grade |
Getting to know and understand the chemical basis of the structure and function of the body.
The basic knowledge of inorganic and organic chemistry and biophysics.
Lecture Biochemistry - clarifying concepts and content. Amino acids and proteins. Peptide bond. Proteins - structure, classification, and the complexity of the structural and functional diversity. The parameters characterizing the properties of the proteins. Enzymes - basic functions. Enzyme inhibitors. Nucleic acids - structure, diversity, function, biosynthesis. The genetic code. Biosynthesis of proteins. Sugars - structure and function in the body. Lipids - complexity and classification, biosynthesis and catabolism of certain lipids. Vitamins - the characteristics and distribution of vitamins. Metabolism of basic concepts. Anabolic and catabolic processes. Coupled reactions. Glycolysis. Krebs cycle. Photosynthesis. Oxidative phosphorylation. Inputs of nitrogen to the biosphere. Chromatographic techniques. Electrophoresis. Basics of spectrophotometry.
Laboratory To acquaint students with the research apparatus used in the biochemical laboratory. Service, maintenance. Discussion of principles regarding the preparation of biochemical reagents. Biochemical calculations. Qualitative and quantitative analysis of carbohydrates. Identification of unknown sugar. Qualitative and quantitative analysis of specific fats. The reaction of saponification of specific fats. Fat numbers. Preparation and analysis of lecithin from egg yolks. Qualitative and quantitative analysis of vitamins. Characteristic reactions of amino acids. Reactions characteristic for selected amino acids. Identification of an unknown amino acid. Quality analysis of proteins. Protein properties in solution. Sacking of egg protein (separation of albumin from globulins). Protein denaturation. Quantitative analysis of proteins. Spectrophotometric analysis. Enzymatic reaction. Inhibitors and activators. Determination of activity and detection of selected enzymes. Acid hydrolysis of nucleic acids. DNA and RNA isolation. Quality analysis of nucleic acids. Pentose detection. Detection of purine and pyrimidine bases. Detection of orthophosphoric acid (V). DNA electrophoresis in agarose gel. Quantitative analysis of nucleic acids. Determination of purity of nucleic acid preparations.
Lecture: giving method - lecture in the form of a multimedia presentation in the classroom;
Laboratory: giving method: discussion on the application of analytical methods; Practical method: lab exercises with the use of selected: biochemical methods, methods for documenting the results and bioinformatics programs.
| Outcome description | Outcome symbols | Methods of verification | The class form |
LECTURE: provided credit is to get positive results from written examination test. Least 50% of scored points are required to get the pass mark credit.
LABORATORY: provided credit is class attendance and getting positive results of the tests (a positive mark above 50% of scored points), and credit of all written reports from performed laboratory experiments. The final mark consists of the average sum of all of the passed partial marks.
1. Berg, J.M, Tymoczko, J.L. , Stryer, L., Biochemia, Wydawnictwo Naukowe PWN, Warszawa, 2005, wydanie IV zmienione.
2. Berg, J.M, Tymoczko, J.L. , Stryer, L., Biochemia, Wydawnictwo Naukowe PWN, Warszawa, 2009, wydanie VI zmienione.
1. P. Kafarski & B. Lejczak, Chemia bioorganiczna, PWN, Warszawa, 1994.
2. J. Staniec, A. Bojarska Ćwiczenia z biochemii dla studentów biologii, Wydaw. Naukowe AP, Kraków, 2001
3. L. Kłyszejko-Stefanowicz Ćwiczenia z biochemii, PWN, Warszawa, 2018.
4. A. Zgirski, R. Gondko Obliczenia biochemiczne , PWN, Warszawa, 2010
Modified by dr hab. inż. Dżamila Bogusławska, prof. UZ (last modification: 25-05-2021 17:23)
