SylabUZ
| Course name | General Genetics |
| Course ID | 13.9-WB-OS2P-GeneOg-S17 |
| Faculty | Faculty of Biological Sciences |
| Field of study | Environmental Protection |
| Education profile | academic |
| Level of studies | First-cycle studies leading to Bachelor's degree |
| Beginning semester | winter term 2021/2022 |
| Semester | 5 |
| ECTS credits to win | 4 |
| Course type | obligatory |
| Teaching language | english |
| Author of syllabus |
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| The class form | Hours per semester (full-time) | Hours per week (full-time) | Hours per semester (part-time) | Hours per week (part-time) | Form of assignment |
| Lecture | 30 | 2 | - | - | Exam |
| Laboratory | 30 | 2 | - | - | Credit with grade |
The objective of the course is to get the student acquired knowledge of the structure and organisation of genetic material and of relations between its structure and function. To get the student acquainted with the basic mechanisms of genetic material expression and the basics of chromosomal theory of inheritance (Mendel's laws, mitosis, meiosis) and exceptions from the rules. To acquire knowledge of mutations and the occurrence of mobile genetic elements. To acquire basic knowledge of population genetics. Within the laboratory classes the student is acquainted with the basic safety rules in a genetic-type laboratory. The student should be able to perform independently simple experiments in the field of classical genetics. The student should be able to solve tasks in genetics as well as to critically analyze and interpret the results of the carried out experiments.
Knowledge of biology, chemistry, biochemistry required within the program of secondary education
Lecture: The subject and the domain of genetics. Watson and Crick's model of DNA and the functions of genetic material. Chemical nature of polynucleotide. The organisation of the genetic material in Prokaryotes and Eukaryotes. Replication and recombination. Basics mechanisms of DNA synthesis. Mechanisms and forms of recombination. Basics of transcription. Detailed transcription mechanism. Operons – basic units of gene expression in bacteria. Transcription termination. The structure and expression of an eukaryotic gene. Chromatin structure. RNA polymerase and its role. Gene promotors – transcription regulation, DNA-protein interactions, translation. Relations between genes and proteins, ribosomes – functions of ribosome protein. Transfer RNA.Genetic code. Translation mechanisms. Chromosomal theory of inheritance and its basis e.g. Mendel's laws as well as mitosis and meiosis. Evidence for chromosomal theory of inheritance; sex related features. Exceptions from Mendel's laws: incomplete dominance, gene epistasis, lethal genes, maternal effect, gene linkage. Chromosomal maps and the notion of linkage groups. Gene mutation and inborn errors of metabolism. Complementation testing – analysis of location of cis-trans alleles. Point and genomic mutations. Mobile genetic elements. Basics of population genetics Hardy-Weinberg Law.
Laboratory: Introduction to genetics - Drosophila melanogaster as an example of the object of genetic tests. Meiosis. Mutants of Drosophila melanogaster. 1st and 2nd Mendel's laws. Interaction between alleles within the same gene. Multiple alleles. Lethal and sub-lethal alleles. Pleiotropic genes. Interaction between non-allelic genes. Epistasis and genetic tasks in it. Inheritance of coupled and sex-related features, sex determination and genetic tasks in it. Gene linkage. Gene Mapping and genetic tasks in it. Complementation. Discussion and interpretation of the results of D. melanogaster mutants.
- Lecture in a form of multimedia presentations
- Practical method – laboratory classes (with the use of binoculars and magnifiers as well as a collection of D. melanogaster mutants).
| Outcome description | Outcome symbols | Methods of verification | The class form |
Verification of learning outcomes and credit conditions:
Lecture – final exam in a written form, which student takes after having obtained a credit for classes. The exam lasts 90 min. and consists of 60 closed questions. Positive grade is granted after obtaining above 39 points (65%) out of 60 points.
Laboratory - The credit is granted after all experiments to be realized within the laboratory program are performed and positively evaluated. The evaluation includes: presence, tests (open and closed) – positive grade is granted after having obtained above 60% of points, the ability to carry out fruit fly crossing. The final grade is the arithmetic mean of partial grades.
1. W.S. Klug & M.R. Cummings, Concepts of Genetics, A Bell &Howell Publishing Company
2 T.A. Brown, Genomes, Garland Science Taylor &Francis Group
Modified by dr Olaf Ciebiera (last modification: 19-05-2021 22:02)
