1. SylabUZ
2. Wydział Nauk Biologicznych
3. semestr zimowy 2022/2023
4. WNB - oferta ERASMUS - Program Erasmus
5. Microbiology with immunology

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Microbiology with immunology - opis przedmiotu

Cel przedmiotu

The acquisition of theoretical and practical knowledge by the student, as a result of which the student should describe: biological and physiological functions of bacteria and fungi; metabolic processes and their regulation in the mentioned organisms; possibilities of using the biological potential of bacteria and fungi in biotechnology; biological properties and regulatory functions of viruses. The aim of the course is also to get to know the structure and biological functions of the immune system. The student should explain the essence and regulatory mechanism of defense reactions conditioned by elements of non-specific and specific immunity mechanisms.
Within the laboratory classes the student should learn the basic principles of safe work in a biological laboratory, master the culture techniques used in microbiology, basic experimental and laboratory techniques used in immunology.

Wymagania wstępne

Zapisz zmiany

Knowledge of basic biology, chemistry, biochemistry.

Zakres tematyczny

Lecture: The place of microorganisms in the world of living organisms. Structure and function of bacterial cell. Metabolic processes in bacteria and mechanisms of their regulation. Genetics of bacteria. Bacteria in biosphere. Interaction of bacteria. Viruses and their biological properties. Fungi and their biological properties. Pathogenic microorganisms for plants, animals and humans. Structure of immune system. Nonspecific immunity. Specific immunity. Immune response of humoral type. Immune response of cellular type. Regulation of immune response. Evolution of immunity.

Laboratory activities: Microscopic observations. Size and shape of microorganisms. Gram staining. Complex staining - structural elements of bacteria. Sterilization. Microbiological media. Culture techniques. Isolating bacteria and obtaining pure cultures. Determination of bacteria number. Effects of physical and chemical factors on bacteria. Methods of studying biochemical properties of bacterial cells. Isolation and observation of immune cells. Antigen-antibody reactions (precipitation, complement fixation, immunofluorescence, immunodiffusion, immunoenzymatic ELISA test). Methods for determining complement. Methods of measuring effector functions of immune cells (migration, adherence, engulfment, intracellular killing, metabolic activity).

Metody kształcenia

- feeding method (lecture in the form of multimedia presentation),

- practical (laboratory exercises using classical and molecular techniques used in microbiological studies)

Efekty uczenia się i metody weryfikacji osiągania efektów uczenia się

Opis efektu	Symbole efektów	Metody weryfikacji	Forma zajęć

Warunki zaliczenia

Lecture - final exam, which the student is admitted on the basis of the prior pass of the laboratory classes. Exam: I term - written form - 50 question test, further deadlines – oral. Exam time - 90 min. Rating - satisfactory - over 60% of all points.

Laboratory - knowledge tests (closed and open) - positive score over 60% of points obtained and practical skills test. Final score is the arithmetic mean of the partial scores.

Literatura podstawowa

1. Basic Practical Microbiology. Society for General Microbiology 2006

2. Essential Microbiology. S. Hogg. John Wiley & Sons Ltd. 2005

3. Medical microbiology. P. Murray, K. Rosenthal, M. Pfaller. Elsevier. 2016

4. Textbook of microbiology. C.K.J. Paniker. Orient Longman. 2005

5. Laboratory Manual and Workbook in Microbiology. J. A. Morello, P. A. Granato, H. E. Mizer. The McGraw−Hill Companies, 2003

Literatura uzupełniająca

1. Microbiology. M. Sattley, M.T. Madigan. John Wiley & Sons, Ltd. 2015

Uwagi

Zmodyfikowane przez dr Sylwia Andrzejczak-Grządko (ostatnia modyfikacja: 08-05-2022 17:45)